منابع مشابه
On the mechanism of spermine transport in liver mitochondria.
Spermine penetrates the mitochondrial matrix at significant rates which increase sharply and non-ohmically with membrane potential. In this respect, spermine uptake is qualitatively similar to that of other cations whose electrophoretic transport has been studied in mitochondria. At 200 mV and 1 mM spermine, the observed rate of spermine uptake was about 7 nmol x mg-1 x min-1, and the rate cons...
متن کاملSpermine binding to submitochondrial particles and activation of adenosine triphosphatase.
Studies on the effects of polyamines on oligomycin-sensitive ATPase activity of ox heart submitochondrial particles showed that, of the polyamines tested, only spermine affected the enzyme activity. Spermine within the physiological concentration range increased the Vmax. of the enzyme, but the Km for ATP was virtually unaffected. Binding studies of [14C]spermine to submitochondrial particles, ...
متن کاملStudies of the acetyl-CoA-binding site of rat liver spermidine/spermine N1-acetyltransferase.
Rat liver spermidine/spermine N1-acetyltransferase was found to be strongly inhibited by the dyes Cibacron F3GA, Coomassie Brilliant Blue and Congo Red. Inhibition was competitive with respect to acetyl-CoA and Ki values of 0.7 microM and 52 microM were determined for Cibacron F3GA and Coomassie Brilliant Blue respectively. The enzyme was strongly retained by columns of Affi-Gel Blue, which con...
متن کاملStudies on the Binding of Concanavalin A to Rat Liver Mitochondria”
The binding of concanavalin A (con A) to intact and detergent-solubilized fractions of rat liver mitochondria has been studied quantitatively using a turbidimetric agglutination assay and by a radioactive binding procedure employing [acefyl-3H]con A. The reaction appears to involve oligosaccharide residues associated with glycoproteins of the mitochondrion since glycosides such as methyl-c-u-D-...
متن کاملEffects of palmitic acid on the binding of insulin 131-I to the rat liver mitochondria.
Effects of palmitic acid, inhibitors, uncouplers, and reduced and oxidized glutathione on the binding of insulin-131! and 131! to the isolated fresh or aged mitochondria were studied. The binding of insulin-1311 to the fresh mitochondria was unexpectedly increased to a greater degree with the exogenous addition of palmitic acid, glutathione, acetaldehyde and 2, 4-dinitrophenol to the medium. Th...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1996
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(96)00140-x